› 3q36

X-ray diffraction
2.5Å resolution

Crystal structure of the 4Fe-4S cluster domain of human DNA primase large subunit

Released:
DOI: 10.2210/pdb3q36/pdb
PDB entry 3q36 coloured by chain, front view.
PDB entry 3q36 coloured by chain, side view.
PDB entry 3q36 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the C-terminal domain of human DNA primase large subunit: implications for the mechanism of the primase-polymerase α switch.
Agarkar VB, Babayeva ND, Pavlov YI, Tahirov TH
Cell Cycle 10 926-31 (2011)
PMID: 21346410

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155975 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49643 (Residues: 266-457; Coverage: 38%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 126.476Å b: 83.724Å c: 47.561Å
α: 90° β: 97.65° γ: 90°
R-values:
R R work R free
0.228 0.228 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

ChSeq: A database of chameleon sequences.
Li et al. (2015)
3 more

8 mentions without citation

Elaborated Action of the Human Primosome.
Baranovskiy et al. (2017)
7 more