› 3qf9

X-ray diffraction
2.2Å resolution

Crystal structure of human proto-oncogene serine threonine kinase (PIM1) in complex with a consensus peptide and a furan-thiazolidinedione ligand

Released:
DOI: 10.2210/pdb3qf9/pdb
PDB entry 3qf9 coloured by chain, front view.
PDB entry 3qf9 coloured by chain, side view.
PDB entry 3qf9 coloured by chain, top view.
Source organisms:
Entry authors: Filippakopoulos P, Bullock AN, Fedorov O, Miduturu CV, von Delft F, Arrowsmith CH, Weigelt J, Edwards A, Bountra C, Grey N, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145762 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase pim-1 Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 35.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11309 (Residues: 1-312; Coverage: 100%)
Gene name: PIM1
Sequence domains: Protein kinase domain
Structure domains:
pimtide Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.59 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NM8 1 x NM8
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P65
Unit cell:
a: 97.843Å b: 97.843Å c: 80.428Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links