› 3qft

X-ray diffraction
1.4Å resolution

Crystal Structure of NADPH-Cytochrome P450 Reductase (FAD/NADPH domain and R457H Mutant)

Released:
DOI: 10.2210/pdb3qft/pdb
PDB entry 3qft coloured by chain, front view.
PDB entry 3qft coloured by chain, side view.
PDB entry 3qft coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for human NADPH-cytochrome P450 oxidoreductase deficiency.
Xia C, Panda SP, Marohnic CC, Martásek P, Masters BS, Kim JJ
Proc Natl Acad Sci U S A 108 13486-91 (2011)
PMID: 21808038

Function and Biology Details

Reaction catalysed: 
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147724 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH--cytochrome P450 reductase Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16435 (Residues: 241-677; Coverage: 65%)
Gene names: CYPOR, POR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP

Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 54.8Å b: 58.624Å c: 130.198Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.227
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Human cytochromes P450 in health and disease.
Nebert et al. (2013)
13 more

2 mentions without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)
1 more