› 3qic

X-ray diffraction
2.2Å resolution

The structure of human glucokinase E339K mutation

Released:
DOI: 10.2210/pdb3qic/pdb
PDB entry 3qic coloured by chain, front view.
PDB entry 3qic coloured by chain, side view.
PDB entry 3qic coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of E339K mutated human glucokinase reveals changes in the ATP binding site.
Liu Q, Shen Y, Liu S, Weng J, Liu J
FEBS Lett 585 1175-9 (2011)
PMID: 21420961

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-4 Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 53.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35557 (Residues: 12-465; Coverage: 98%)
Gene name: GCK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLC 1 x GLC
Ligand GOL 6 x GOL
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 80.74Å b: 80.74Å c: 178.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.25
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Homotropic allosteric regulation in monomeric mammalian glucokinase.
Larion et al. (2012)
1 more

3 mentions without citation

ProteinsPlus: a web portal for structure analysis of macromolecules.
Fährrolfes et al. (2017)
2 more