› 3qoq

X-ray diffraction
3.1Å resolution

Crystal Structure of the Transcription Factor AmrZ in Complex with the 18 Base Pair amrZ1 Binding Site

Released:
DOI: 10.2210/pdb3qoq/pdb
PDB entry 3qoq coloured by chain, front view.
PDB entry 3qoq coloured by chain, side view.
PDB entry 3qoq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes.
Pryor EE, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, Hollis T
PLoS Pathog 8 e1002648 (2012)
PMID: 22511872

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116779 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor AmrZ Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 69 amino acids
Theoretical weight: 7.91 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XCY4 (Residues: 1-66; Coverage: 61%)
Gene names: PA3385, amrZ
Sequence domains: Arc-like DNA binding domain
Structure domains: Met repressor-like
DNA (5'-D(*AP*CP*TP*GP*GP*CP*AP*AP*AP*AP*CP*GP*CP*CP*GP*GP*CP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*CP*GP*GP*CP*GP*TP*TP*TP*TP*GP*CP*CP*AP*GP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 18 nucleotides
Theoretical weight: 5.51 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I422
Unit cell:
a: 129.465Å b: 129.465Å c: 152.554Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.261 0.295
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Biogenesis of Pseudomonas aeruginosa type IV pili and regulation of their function.
Leighton et al. (2015)
5 more

5 mentions without citation

Microbial alginate production, modification and its applications.
Hay et al. (2013)
4 more