› 3qpc

X-ray diffraction
0.98Å resolution

Structure of Fusarium Solani Cutinase expressed in Pichia pastoris, crystallized in the presence of Paraoxon

Released:
DOI: 10.2210/pdb3qpc/pdb
PDB entry 3qpc coloured by chain, front view.
PDB entry 3qpc coloured by chain, side view.
PDB entry 3qpc coloured by chain, top view.
Source organism: Fusarium solani
Entry authors: Lu A, Gosser Y, Montclare JK, Liu Z, Kong X

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase 1 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 20.58 KDa
Source organism: Fusarium solani
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00590 (Residues: 32-228; Coverage: 92%)
Gene names: CUT1, CUTA
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MIR 1 x MIR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P212121
Unit cell:
a: 36.8Å b: 64.385Å c: 70.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.163
Expression system: Komagataella pastoris

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