› 3qr3

X-ray diffraction
2.05Å resolution

Crystal Structure of Cel5A (EG2) from Hypocrea jecorina (Trichoderma reesei)

Released:
DOI: 10.2210/pdb3qr3/pdb
PDB entry 3qr3 coloured by chain, front view.
PDB entry 3qr3 coloured by chain, side view.
PDB entry 3qr3 coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
A structural study of Hypocrea jecorina Cel5A.
Lee TM, Farrow MF, Arnold FH, Mayo SL
Protein Sci 20 1935-40 (2011)
PMID: 21898652

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase EG-II Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 36.59 KDa
Source organism: Trichoderma reesei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07982 (Residues: 92-418; Coverage: 82%)
Gene name: egl2
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand SO4 14 x SO4
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 82.953Å b: 84.593Å c: 90.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.205
Expression system: Escherichia coli BL21

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Citations

2 review citations

Cellulases for biomass degradation: comparing recombinant cellulase expression platforms.
Garvey et al. (2013)
1 more

9 mentions without citation

A structural study of Hypocrea jecorina Cel5A.
Lee et al. (2011)
8 more