› 3qrr

X-ray diffraction
3.1Å resolution

Structure of Thermus Thermophilus Cse3 bound to an RNA representing a product complex

Released:
DOI: 10.2210/pdb3qrr/pdb
PDB entry 3qrr coloured by chain, front view.
PDB entry 3qrr coloured by chain, side view.
PDB entry 3qrr coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Recognition and maturation of effector RNAs in a CRISPR interference pathway.
Gesner EM, Schellenberg MJ, Garside EL, George MM, Macmillan AM
Nat Struct Mol Biol 18 688-92 (2011)
PMID: 21572444

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-176183 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cse3 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53WG9 (Residues: 1-211; Coverage: 100%)
Gene names: TTHB192, cas6, cse3
Sequence domains: CRISPR associated protein
Structure domains:
RNA (5'-R(*GP*UP*CP*CP*CP*CP*AP*CP*GP*CP*GP*UP*GP*UP*GP*GP*GP*(23G))-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.86 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 23G 1 x 23G

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: I222
Unit cell:
a: 62.17Å b: 68.747Å c: 155.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.3
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

29 review citations

RNA-guided genetic silencing systems in bacteria and archaea.
Wiedenheft et al. (2012)
28 more

1 mention without citation

Structural Principles of CRISPR RNA Processing.
Li H. (2015)