› 3qvp

X-ray diffraction
1.2Å resolution

Crystal structure of glucose oxidase for space group C2221 at 1.2 A resolution

Released:
DOI: 10.2210/pdb3qvp/pdb
PDB entry 3qvp coloured by chain, front view.
PDB entry 3qvp coloured by chain, side view.
PDB entry 3qvp coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Probing oxygen activation sites in two flavoprotein oxidases using chloride as an oxygen surrogate.
Kommoju PR, Chen ZW, Bruckner RC, Mathews FS, Jorns MS
Biochemistry 50 5521-34 (2011)
PMID: 21568312

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose + O(2) = D-glucono-1,5-lactone + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146428 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucose oxidase Chain: A
Molecule details ›
Chain: A
Length: 583 amino acids
Theoretical weight: 63.33 KDa
Source organism: Aspergillus niger
Expression system: Not provided
UniProt:
  • Canonical: P13006 (Residues: 23-605; Coverage: 100%)
Gene name: gox
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 3 x NAG
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 69.56Å b: 119.147Å c: 142.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.178 0.188
Expression system: Not provided

Quick links

Citations

20 citation in other articles

The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate.
Tan et al. (2013)
19 more

12 mentions without citation

Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.
Bauer et al. (2022)
11 more