› 3r7w

X-ray diffraction
2.77Å resolution

Crystal Structure of Gtr1p-Gtr2p complex

Released:
DOI: 10.2210/pdb3r7w/pdb
PDB entry 3r7w coloured by chain, front view.
PDB entry 3r7w coloured by chain, side view.
PDB entry 3r7w coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the Gtr1p-Gtr2p complex reveals new insights into the amino acid-induced TORC1 activation.
Gong R, Li L, Liu Y, Wang P, Yang H, Wang L, Cheng J, Guan KL, Xu Y
Genes Dev 25 1668-73 (2011)
PMID: 21816923

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156747 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding protein GTR1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 307 amino acids
Theoretical weight: 36.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00582 (Residues: 8-310; Coverage: 98%)
Gene names: GTR1, YM7056.05, YML121W
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains:
GTP-binding protein GTR2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 331 amino acids
Theoretical weight: 38.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53290 (Residues: 11-341; Coverage: 97%)
Gene names: GTR2, YGR163W
Sequence domains: Gtr1/RagA G protein conserved region
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GNP 4 x GNP
Ligand MG 4 x MG
1 modified residue:
Ligand MSE 46 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 68.908Å b: 148.439Å c: 98.508Å
α: 90° β: 100.61° γ: 90°
R-values:
R R work R free
0.238 0.236 0.283
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Target of rapamycin (TOR) in nutrient signaling and growth control.
Loewith et al. (2011)
22 more

12 mentions without citation

The Architecture of the Rag GTPase Signaling Network.
Nicastro et al. (2017)
11 more