› 3ret

X-ray diffraction
1.79Å resolution

Salicylate and Pyruvate Bound Structure of the Isochorismate-Pyruvate Lyase K42E Mutant from Pseudomonas aerugionsa

Released:
DOI: 10.2210/pdb3ret/pdb
PDB entry 3ret coloured by chain, front view.
PDB entry 3ret coloured by chain, side view.
PDB entry 3ret coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
pH Dependence of catalysis by Pseudomonas aeruginosa isochorismate-pyruvate lyase: implications for transition state stabilization and the role of lysine 42.
Olucha J, Ouellette AN, Luo Q, Lamb AL
Biochemistry 50 7198-207 (2011)
PMID: 21751784

Function and Biology Details

Reactions catalysed: 
Isochorismate = salicylate + pyruvate
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isochorismate pyruvate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q51507 (Residues: 1-101; Coverage: 100%)
Gene names: PA4230, pchB
Sequence domains: Chorismate mutase type II
Structure domains: Chorismate mutase

Ligands and Environments

2 bound ligands:
Ligand SAL 2 x SAL
Ligand PYR 2 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 46.144Å b: 57.313Å c: 60.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.208 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study.
Ronnebaum et al. (2018)
5 other articles

1 mention without citation

pH Dependence of catalysis by Pseudomonas aeruginosa isochorismate-pyruvate lyase: implications for transition state stabilization and the role of lysine 42.
Olucha et al. (2011)