› 3rgp

X-ray diffraction
1.88Å resolution

Structural and Kinetic Analysis of the Beef liver Catalase complexed with Nitric Oxide

Released:
DOI: 10.2210/pdb3rgp/pdb
PDB entry 3rgp coloured by chain, front view.
PDB entry 3rgp coloured by chain, side view.
PDB entry 3rgp coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Interaction of nitric oxide with catalase: structural and kinetic analysis.
Purwar N, McGarry JM, Kostera J, Pacheco AA, Schmidt M
Biochemistry 50 4491-503 (2011)
PMID: 21524057

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 499 amino acids
Theoretical weight: 56.88 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00432 (Residues: 4-502; Coverage: 95%)
Gene name: CAT
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:
Ligand NO 4 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 86.11Å b: 139.94Å c: 228.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.214

Quick links

Citations

9 review citations

Classical catalase: ancient and modern.
Nicholls P. (2012)
8 more

5 mentions without citation

Hydrogen sulfide activation in hemeproteins: the sulfheme scenario.
Ríos-González et al. (2014)
4 more