› 3ri1

X-ray diffraction
2.1Å resolution

Crystal structure of the catalytic domain of FGFR2 kinase in complex with ARQ 069

Released:
DOI: 10.2210/pdb3ri1/pdb
PDB entry 3ri1 coloured by chain, front view.
PDB entry 3ri1 coloured by chain, side view.
PDB entry 3ri1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel mode of protein kinase inhibition exploiting hydrophobic motifs of autoinhibited kinases: discovery of ATP-independent inhibitors of fibroblast growth factor receptor.
Eathiraj S, Palma R, Hirschi M, Volckova E, Nakuci E, Castro J, Chen CR, Chan TC, France DS, Ashwell MA
J Biol Chem 286 20677-87 (2011)
PMID: 21454610

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor receptor 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 35.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21802 (Residues: 458-768; Coverage: 39%)
Gene names: BEK, FGFR2, KGFR, KSAM
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand 3RH 2 x 3RH
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 105.794Å b: 119.581Å c: 63.311Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.247
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Small-molecule inhibitors of the receptor tyrosine kinases: promising tools for targeted cancer therapies.
Hojjat-Farsangi M. (2014)
2 more

8 mentions without citation

A novel mode of protein kinase inhibition exploiting hydrophobic motifs of autoinhibited kinases: discovery of ATP-independent inhibitors of fibroblast growth factor receptor.
Eathiraj et al. (2011)
7 more