› 3rj8

X-ray diffraction
2.4Å resolution

Crystal structure of carbohydrate oxidase from Microdochium nivale

Released:
DOI: 10.2210/pdb3rj8/pdb
PDB entry 3rj8 coloured by chain, front view.
PDB entry 3rj8 coloured by chain, side view.
PDB entry 3rj8 coloured by chain, top view.
Source organism: Microdochium nivale
Entry authors: Duskova J, Skalova T, Stepankova A, Koval T, Hasek J, Ostergaard LH, Fuglsang CC, Kolenko P, Dohnalek J

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose + O(2) = D-glucono-1,5-lactone + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Carbohydrate oxidase Chain: A
Molecule details ›
Chain: A
Length: 473 amino acids
Theoretical weight: 52.47 KDa
Source organism: Microdochium nivale
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: I1SB12 (Residues: 23-495; Coverage: 100%)
Gene name: MnCO
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ZN 3 x ZN
Ligand K 1 x K
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 133.71Å b: 56.96Å c: 87.05Å
α: 90° β: 95.85° γ: 90°
R-values:
R R work R free
0.156 0.155 not available
Expression system: Aspergillus oryzae

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