› 3rn5

X-ray diffraction
2.5Å resolution

Structural basis of cytosolic DNA recognition by innate immune receptors

Released:
DOI: 10.2210/pdb3rn5/pdb
PDB entry 3rn5 coloured by chain, front view.
PDB entry 3rn5 coloured by chain, side view.
PDB entry 3rn5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the HIN domain:DNA complexes reveal ligand binding and activation mechanisms of the AIM2 inflammasome and IFI16 receptor.
Jin T, Perry A, Jiang J, Smith P, Curry JA, Unterholzner L, Jiang Z, Horvath G, Rathinam VA, Johnstone RW, Hornung V, Latz E, Bowie AG, Fitzgerald KA, Xiao TS
Immunity 36 561-71 (2012)
PMID: 22483801

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116834 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Interferon-inducible protein AIM2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14862 (Residues: 144-343; Coverage: 58%)
Gene name: AIM2
Sequence domains: HIN-200/IF120x domain
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(*CP*CP*AP*TP*CP*AP*AP*AP*GP*AP*GP*AP*GP*AP*AP*AP*GP*AP*G)-3') Chains: K, M
Molecule details ›
Chains: K, M
Length: 19 nucleotides
Theoretical weight: 5.9 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*CP*TP*CP*TP*TP*TP*CP*TP*CP*TP*CP*TP*TP*TP*GP*AP*TP*G)-3') Chains: L, N
Molecule details ›
Chains: L, N
Length: 19 nucleotides
Theoretical weight: 5.74 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 62.36Å b: 136.36Å c: 77.04Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.202 0.201 0.241
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

179 review citations

Activation and regulation of the inflammasomes.
Latz et al. (2013)
178 more

8 mentions without citation

AIM2 inflammasome activation and regulation: A structural perspective.
Wang et al. (2017)
7 more