› 3ron

X-ray diffraction
2.19Å resolution

Crystal Structure and Hemolytic Activity of the Cyt1Aa Toxin from Bacillus thuringiensis subsp. israelensis

Released:
DOI: 10.2210/pdb3ron/pdb
PDB entry 3ron coloured by chain, front view.
PDB entry 3ron coloured by chain, side view.
PDB entry 3ron coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis
Primary publication:
Cyt1Aa toxin: crystal structure reveals implications for its membrane-perforating function.
Cohen S, Albeck S, Ben-Dov E, Cahan R, Firer M, Zaritsky A, Dym O
J Mol Biol 413 804-14 (2011)
PMID: 21959261

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-1Aa cytolytic delta-endotoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.35 KDa
Source organism: Bacillus thuringiensis serovar israelensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A382 (Residues: 1-249; Coverage: 100%)
Gene names: cyt1Aa, cytA
Sequence domains: Bacillus thuringiensis toxin
Structure domains: Delta-endotoxin CytB-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 33.895Å b: 65.822Å c: 176.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.261
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins.
Ben-Dov E. (2014)
3 more

9 mentions without citation

Bacillus thuringiensis toxins: an overview of their biocidal activity.
Palma et al. (2014)
8 more