› 3rub

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE UNACTIVATED FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM TOBACCO REFINED AT 2.0-ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb3rub/pdb
PDB entry 3rub coloured by chain, front view.
PDB entry 3rub coloured by chain, side view.
PDB entry 3rub coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
Crystal structure of the unactivated form of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco refined at 2.0-A resolution.
Curmi PM, Cascio D, Sweet RM, Eisenberg D, Schreuder H
J Biol Chem 267 16980-9 (1992)
PMID: 1512238

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133653 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chain: L
Molecule details ›
Chain: L
Length: 477 amino acids
Theoretical weight: 53.04 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P00876 (Residues: 1-477; Coverage: 100%)
Gene names: rbcA, rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small subunit, chloroplastic Chain: S
Molecule details ›
Chain: S
Length: 123 amino acids
Theoretical weight: 14.57 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P69249 (Residues: 58-180; Coverage: 68%)
Gene name: RBCS
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: Ribulose bisphosphate carboxylase, small subunit

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand ASN 1 x ASN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 148.7Å b: 148.7Å c: 137.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

Checking your imagination: applications of the free R value.
Kleywegt et al. (1996)
3 more

4 mentions without citation

Analysis on conservation of disulphide bonds and their structural features in homologous protein domain families.
Thangudu et al. (2008)
3 more