› 3s67

X-ray diffraction
2.26Å resolution

Crystal structure of V57P mutant of human cystatin C

Released:
DOI: 10.2210/pdb3s67/pdb
PDB entry 3s67 coloured by chain, front view.
PDB entry 3s67 coloured by chain, side view.
PDB entry 3s67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of V57D and V57P mutants of human cystatin C, an amyloidogenic protein.
Orlikowska M, Szymańska A, Borek D, Otwinowski Z, Skowron P, Jankowska E
Acta Crystallogr D Biol Crystallogr 69 577-86 (2013)
PMID: 23519666

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133889 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-C Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01034 (Residues: 27-146; Coverage: 100%)
Gene name: CST3
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

4 bound ligands:
Ligand PEG 3 x PEG
Ligand IMD 2 x IMD
Ligand ACT 3 x ACT
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I432
Unit cell:
a: 140.135Å b: 140.135Å c: 140.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.229
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

The domain swapping of human cystatin C induced by synchrotron radiation.
Taube et al. (2019)
2 more

3 mentions without citation

High Molecular Weight Kininogen: A Review of the Structural Literature.
Ponczek MB. (2021)
2 more