› 3s8q

X-ray diffraction
2.1Å resolution

Crystal structure of the R-M controller protein C.Esp1396I OL operator complex

Released:
DOI: 10.2210/pdb3s8q/pdb
PDB entry 3s8q coloured by chain, front view.
PDB entry 3s8q coloured by chain, side view.
PDB entry 3s8q coloured by chain, top view.
Source organism: Enterobacter sp. RFL1396
Primary publication:
Recognition of dual symmetry by the controller protein C.Esp1396I based on the structure of the transcriptional activation complex.
McGeehan JE, Ball NJ, Streeter SD, Thresh SJ, Kneale GG
Nucleic Acids Res 40 4158-67 (2012)
PMID: 22210861

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116866 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.52 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*TP*GP*TP*GP*AP*CP*TP*TP*AP*TP*AP*GP*TP*CP*CP*GP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*CP*GP*GP*AP*CP*TP*AP*TP*AP*AP*GP*TP*CP*AP*CP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Unit cell:
a: 44.316Å b: 61.546Å c: 113.687Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

From biophysics to 'omics and systems biology.
Djordjevic et al. (2019)
4 other articles

3 mentions without citation

Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins.
Hynes et al. (2018)
2 more