› 3s8r

X-ray diffraction
2.5Å resolution

Crystal Structures of Glutaryl 7-Aminocephalosporanic Acid Acylase: Insight into Autoproteolytic Activation

Released:
DOI: 10.2210/pdb3s8r/pdb
PDB entry 3s8r coloured by chain, front view.
PDB entry 3s8r coloured by chain, side view.
PDB entry 3s8r coloured by chain, top view.
Source organism: Pseudomonas sp. SY-77-1
Primary publication:
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH
Biochemistry 42 4084-93 (2003)
PMID: 12680762

Function and Biology Details

Reaction catalysed: 
(7R)-7-(4-carboxybutanamido)cephalosporanate + H(2)O = (7R)-7-aminocephalosporanate + glutarate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaryl-7-aminocephalosporanic-acid acylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 697 amino acids
Theoretical weight: 77.58 KDa
Source organism: Pseudomonas sp. SY-77-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P07662 (Residues: 30-720; Coverage: 100%)
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: C2
Unit cell:
a: 225.386Å b: 68.806Å c: 112.756Å
α: 90° β: 97.51° γ: 90°
R-values:
R R work R free
0.156 0.154 0.2
Expression system: Escherichia coli

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Citations

2 review citations

Cephalosporin C acylase: dream and(/or) reality.
Pollegioni et al. (2013)
1 more