› 3scu

X-ray diffraction
1.58Å resolution

Crystal Structure of Rice BGlu1 E386G Mutant Complexed with Cellopentaose

Released:
DOI: 10.2210/pdb3scu/pdb
PDB entry 3scu coloured by chain, front view.
PDB entry 3scu coloured by chain, side view.
PDB entry 3scu coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Entry authors: Pengthaisong S, Withers SG, Kuaprasert B, Ketudat Cairns JR

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-glucosidase 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 481 amino acids
Theoretical weight: 54.66 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75I93 (Residues: 29-504; Coverage: 100%)
Gene names: BGLU1, BGLU7, LOC_Os03g49600, OSJNBa0004L11.16, Os03g0703000
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 2 x SO4
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 79.468Å b: 100.782Å c: 127.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.192
Expression system: Escherichia coli

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