› 3sea

X-ray diffraction
2Å resolution

Structure of Rheb-Y35A mutant in GDP- and GMPPNP-bound forms

Released:
DOI: 10.2210/pdb3sea/pdb
PDB entry 3sea coloured by chain, front view.
PDB entry 3sea coloured by chain, side view.
PDB entry 3sea coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An autoinhibited noncanonical mechanism of GTP hydrolysis by Rheb maintains mTORC1 homeostasis.
Mazhab-Jafari MT, Marshall CB, Ishiyama N, Ho J, Di Palma V, Stambolic V, Ikura M
Structure 20 1528-39 (2012)
PMID: 22819219

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rheb Chains: A, B
Molecule details ›
Chains: A, B
Length: 167 amino acids
Theoretical weight: 18.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15382 (Residues: 3-169; Coverage: 91%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand GDP 1 x GDP
Ligand GNP 1 x GNP
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Unit cell:
a: 57.247Å b: 69.873Å c: 79.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.162 0.214
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Regulation of mTORC1 by PI3K signaling.
Dibble et al. (2015)
6 more

3 mentions without citation

Editorial for the Special Issue of Biophysical Reviews focused on the Biophysical Society of Japan with select scientific content from the 57th BSJ annual meeting, Miyazaki, Japan.
Komatsuzaki et al. (2020)
2 more