› 3sf4

X-ray diffraction
2.6Å resolution

Crystal structure of the complex between the conserved cell polarity proteins Inscuteable and LGN

Released:
DOI: 10.2210/pdb3sf4/pdb
PDB entry 3sf4 coloured by chain, front view.
PDB entry 3sf4 coloured by chain, side view.
PDB entry 3sf4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for interaction between the conserved cell polarity proteins Inscuteable and Leu-Gly-Asn repeat-enriched protein (LGN).
Yuzawa S, Kamakura S, Iwakiri Y, Hayase J, Sumimoto H
Proc Natl Acad Sci U S A 108 19210-5 (2011)
PMID: 22074847

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160498 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
G-protein-signaling modulator 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 406 amino acids
Theoretical weight: 44.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P81274 (Residues: 20-421; Coverage: 59%)
Gene names: GPSM2, LGN
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
Protein inscuteable homolog Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 52 amino acids
Theoretical weight: 5.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1MX18 (Residues: 70-116; Coverage: 8%)
Gene name: INSC
Sequence domains: Inscuteable LGN-binding domain
Structure domains: Inscuteable LGN-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P41212
Unit cell:
a: 124.815Å b: 124.815Å c: 233.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.262
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Spindle orientation in mammalian cerebral cortical development.
Lancaster et al. (2012)
12 more

9 mentions without citation

On the inscrutable role of Inscuteable: structural basis and functional implications for the competitive binding of NuMA and Inscuteable to LGN.
Mapelli et al. (2012)
8 more