› 3sfw

X-ray diffraction
1.73Å resolution

Crystal structure of dihydropyrimidinase from Brevibacillus agri NCHU1002

Released:
DOI: 10.2210/pdb3sfw/pdb
PDB entry 3sfw coloured by chain, front view.
PDB entry 3sfw coloured by chain, side view.
PDB entry 3sfw coloured by chain, top view.
Source organism: Brevibacillus agri
Entry authors: Wang WC, Wu HM

Function and Biology Details

Reaction catalysed: 
5,6-dihydrouracil + H(2)O = 3-ureidopropanoate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 461 amino acids
Theoretical weight: 50.58 KDa
Source organism: Brevibacillus agri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q846U5 (Residues: 1-461; Coverage: 100%)
Gene name: pydB
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand ZN 4 x ZN
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P21212
Unit cell:
a: 65.807Å b: 109.112Å c: 140.984Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.19
Expression system: Escherichia coli

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