› 3shf

X-ray diffraction
3.55Å resolution

Crystal structure of the R265S mutant of full-length murine Apaf-1

Released:
DOI: 10.2210/pdb3shf/pdb
PDB entry 3shf coloured by chain, front view.
PDB entry 3shf coloured by chain, side view.
PDB entry 3shf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of full-length Apaf-1: how the death signal is relayed in the mitochondrial pathway of apoptosis.
Reubold TF, Wohlgemuth S, Eschenburg S
Structure 19 1074-83 (2011)
PMID: 21827944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand GBL 2 x GBL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 72.88Å b: 111.82Å c: 244.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.305
Expression system: Spodoptera frugiperda

Quick links

Citations

23 review citations

Regulation of the intrinsic apoptosis pathway by reactive oxygen species.
Wu et al. (2013)
22 more

1 mention without citation

Crystal structure of the WD40 domain dimer of LRRK2.
Zhang et al. (2019)