› 3sxl

X-ray diffraction
2.7Å resolution

SEX-LETHAL RNA RECOGNITION DOMAINS 1 AND 2 FROM DROSOPHILA MELANOGASTER

Released:
DOI: 10.2210/pdb3sxl/pdb
PDB entry 3sxl coloured by chain, front view.
PDB entry 3sxl coloured by chain, side view.
PDB entry 3sxl coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal.
Crowder SM, Kanaar R, Rio DC, Alber T
Proc Natl Acad Sci U S A 96 4892-7 (1999)
PMID: 10220389

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein sex-lethal Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 184 amino acids
Theoretical weight: 21.03 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19339 (Residues: 113-294; Coverage: 51%)
Gene names: CG43770, Sx1, Sxl
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21
Unit cell:
a: 50.91Å b: 124.47Å c: 50.93Å
α: 90° β: 120° γ: 90°
R-values:
R R work R free
0.221 0.221 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Mechanisms of alternative pre-messenger RNA splicing.
Black DL. (2003)
4 more

5 mentions without citation

Absence of interdomain contacts in the crystal structure of the RNA recognition motifs of Sex-lethal.
Crowder et al. (1999)
4 more