› 3t6c

X-ray diffraction
1.6Å resolution

Crystal structure of an enolase from pantoea ananatis (efi target efi-501676) with bound d-gluconate and mg

Released:
DOI: 10.2210/pdb3t6c/pdb
PDB entry 3t6c coloured by chain, front view.
PDB entry 3t6c coloured by chain, side view.
PDB entry 3t6c coloured by chain, top view.
Source organism: Pantoea ananatis LMG 20103
Entry authors: Vetting MW, Toro R, Bhosle R, Wasserman SR, Morisco LL, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member RspA Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 48.87 KDa
Source organism: Pantoea ananatis LMG 20103
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4GJ14 (Residues: 1-417; Coverage: 100%)
Gene names: PANA_0592, rspA
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand GCO 2 x GCO
Ligand CL 1 x CL
Ligand EDO 9 x EDO
Ligand UNX 2 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P4212
Unit cell:
a: 130.79Å b: 130.79Å c: 104.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.139 0.168
Expression system: Escherichia coli BL21(DE3)

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