› 3t6g

X-ray diffraction
2.5Å resolution

Structure of the complex between NSP3 (SHEP1) and p130Cas

Released:
DOI: 10.2210/pdb3t6g/pdb
PDB entry 3t6g coloured by chain, front view.
PDB entry 3t6g coloured by chain, side view.
PDB entry 3t6g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
NSP-Cas protein structures reveal a promiscuous interaction module in cell signaling.
Mace PD, Wallez Y, Dobaczewska MK, Lee JJ, Robinson H, Pasquale EB, Riedl SJ
Nat Struct Mol Biol 18 1381-7 (2011)
PMID: 22081014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157583 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SH2 domain-containing protein 3C Chains: A, C
Molecule details ›
Chains: A, C
Length: 331 amino acids
Theoretical weight: 37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N5H7 (Residues: 539-860; Coverage: 37%)
Gene names: NSP3, SH2D3C, UNQ272/PRO309/PRO34088
Sequence domains: RasGEF domain
Structure domains: Ras guanine-nucleotide exchange factors catalytic domain
Breast cancer anti-estrogen resistance protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 229 amino acids
Theoretical weight: 25.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56945 (Residues: 645-870; Coverage: 26%)
Gene names: BCAR1, CAS, CASS1, CRKAS
Sequence domains: Crk-Associated Substrate C-terminal domain
Structure domains: Alpha-catenin/vinculin-like

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I41
Unit cell:
a: 171.883Å b: 171.883Å c: 78.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.266
Expression system: Escherichia coli

Quick links

Citations

3 review citations

p130Cas: a key signalling node in health and disease.
Barrett et al. (2013)
2 more

6 mentions without citation

Association of the breast cancer antiestrogen resistance protein 1 (BCAR1) and BCAR3 scaffolding proteins in cell signaling and antiestrogen resistance.
Wallez et al. (2014)
5 more