› 3ta9

X-ray diffraction
3Å resolution

beta-Glucosidase A from the halothermophile H. orenii

Released:
DOI: 10.2210/pdb3ta9/pdb
PDB entry 3ta9 coloured by chain, front view.
PDB entry 3ta9 coloured by chain, side view.
PDB entry 3ta9 coloured by chain, top view.
Source organism: Halothermothrix orenii H 168
Primary publication:
Expression, purification and preliminary crystallographic analysis of the recombinant β-glucosidase (BglA) from the halothermophile Halothermothrix orenii.
Kori LD, Hofmann A, Patel BK
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 111-3 (2011)
PMID: 21206038

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 458 amino acids
Theoretical weight: 53.16 KDa
Source organism: Halothermothrix orenii H 168
Expression system: Escherichia coli
UniProt:
  • Canonical: B8CYA8 (Residues: 1-451; Coverage: 100%)
Gene name: Hore_15280
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 88.504Å b: 99.577Å c: 109.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.191 0.266
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Engineering a thermostable Halothermothrix orenii β-glucosidase for improved galacto-oligosaccharide synthesis.
Hassan et al. (2016)
2 more

5 mentions without citation

Biochemical and structural characterization of a thermostable β-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.
Hassan et al. (2015)
4 more