› 3tg1

X-ray diffraction
2.71Å resolution

Crystal structure of p38alpha in complex with a MAPK docking partner

Released:
DOI: 10.2210/pdb3tg1/pdb
PDB entry 3tg1 coloured by chain, front view.
PDB entry 3tg1 coloured by chain, side view.
PDB entry 3tg1 coloured by chain, top view.
Source organisms:
Primary publication:
A distinct interaction mode revealed by the crystal structure of the kinase p38α with the MAPK binding domain of the phosphatase MKP5.
Zhang YY, Wu JW, Wang ZX
Sci Signal 4 ra88 (2011)
PMID: 22375048

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155656 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 43.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47811 (Residues: 1-360; Coverage: 100%)
Gene names: Crk1, Csbp1, Csbp2, Mapk14
Sequence domains: Protein kinase domain
Structure domains:
Dual specificity protein phosphatase 10 Chain: B
Molecule details ›
Chain: B
Length: 158 amino acids
Theoretical weight: 18.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6W6 (Residues: 139-288; Coverage: 31%)
Gene names: DUSP10, MKP5
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 72.428Å b: 72.428Å c: 226.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.311 0.216 0.26
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Dual-specificity MAP kinase phosphatases (MKPs): shaping the outcome of MAP kinase signalling.
Caunt et al. (2013)
11 more

1 mention without citation

Molecular basis of MAP kinase regulation.
Peti et al. (2013)