› 3tgl

X-ray diffraction
1.9Å resolution

STRUCTURE AND MOLECULAR MODEL REFINEMENT OF RHIZOMUCOR MIEHEI TRIACYLGLYCERIDE LIPASE: A CASE STUDY OF THE USE OF SIMULATED ANNEALING IN PARTIAL MODEL REFINEMENT

Released:
DOI: 10.2210/pdb3tgl/pdb
PDB entry 3tgl coloured by chain, front view.
PDB entry 3tgl coloured by chain, side view.
PDB entry 3tgl coloured by chain, top view.
Source organism: Rhizomucor miehei
Primary publication:
STRUCTURE AND MOLECULAR-MODEL REFINEMENT OF RHIZOMUCOR-MIEHEI TRIACYLGLYCERIDE LIPASE - A CASE-STUDY OF THE USE OF SIMULATED ANNEALING IN PARTIAL MODEL REFINEMENT.
Brzozowski AM, Derewenda ZS, Dodson EJ, Dodson GG, Turkenburg JP
Acta Crystallogr., B 48 307-319 (1992)

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 29.59 KDa
Source organism: Rhizomucor miehei
Expression system: Not provided
UniProt:
  • Canonical: P19515 (Residues: 95-363; Coverage: 79%)
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 71.6Å b: 75Å c: 55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.129 not available not available
Expression system: Not provided

Quick links

Citations

30 mentions without citation

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
29 more