› 3thm

X-ray diffraction
2.1Å resolution

Crystal structure of Fas receptor extracellular domain in complex with Fab EP6b_B01

Released:
DOI: 10.2210/pdb3thm/pdb
PDB entry 3thm coloured by chain, front view.
PDB entry 3thm coloured by chain, side view.
PDB entry 3thm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency.
Chodorge M, Züger S, Stirnimann C, Briand C, Jermutus L, Grütter MG, Minter RR
Cell Death Differ 19 1187-95 (2012)
PMID: 22261618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210891 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Fab EP6b_B01, light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab EP6b_B01, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Tumor necrosis factor receptor superfamily member 6 Chain: F
Molecule details ›
Chain: F
Length: 156 amino acids
Theoretical weight: 17.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25445 (Residues: 17-172; Coverage: 47%)
Gene names: APT1, FAS, FAS1, TNFRSF6
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 94.51Å b: 94.51Å c: 139.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.221
Expression systems:
  • Cricetulus griseus
  • Escherichia coli

Quick links

Citations

14 review citations

Next generation antibody drugs: pursuit of the 'high-hanging fruit'.
Carter et al. (2018)
13 more

5 mentions without citation

A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency.
Chodorge et al. (2012)
4 more