› 3tj6

X-ray diffraction
2.76Å resolution

human vinculin head domain (Vh1, residues 1-258) in complex with the vinculin binding site of the surface cell antigen 4 (sca4-VBS-C; residues 812-835) from Rickettsia rickettsii

Released:
DOI: 10.2210/pdb3tj6/pdb
PDB entry 3tj6 coloured by chain, front view.
PDB entry 3tj6 coloured by chain, side view.
PDB entry 3tj6 coloured by chain, top view.
Source organisms:
Primary publication:
The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin.
Park H, Lee JH, Gouin E, Cossart P, Izard T
J Biol Chem 286 35096-103 (2011)
PMID: 21841197

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-109122 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18206 (Residues: 1-257; Coverage: 23%)
Gene name: VCL
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like
Antigenic heat-stable 120 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.62 KDa
Source organism: Rickettsia rickettsii str. Iowa
Expression system: Escherichia coli
UniProt:
  • Canonical: B0BXR4 (Residues: 812-834; Coverage: 2%)
Gene name: RrIowa_0797

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I213
Unit cell:
a: 141.373Å b: 141.373Å c: 141.373Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.235
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Engineering of obligate intracellular bacteria: progress, challenges and paradigms.
McClure et al. (2017)
11 more

1 mention without citation

The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin.
Park et al. (2011)