› 3tjj

X-ray diffraction
1.91Å resolution

Crystal structure of human peroxiredoxin IV C245A mutant in sulfenylated form

Released:
DOI: 10.2210/pdb3tjj/pdb
PDB entry 3tjj coloured by chain, front view.
PDB entry 3tjj coloured by chain, side view.
PDB entry 3tjj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of reduced and of oxidized peroxiredoxin IV enzyme reveals a stable oxidized decamer and a non-disulfide-bonded intermediate in the catalytic cycle.
Cao Z, Tavender TJ, Roszak AW, Cogdell RJ, Bulleid NJ
J Biol Chem 286 42257-42266 (2011)
PMID: 21994946

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin-4 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 254 amino acids
Theoretical weight: 28.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13162 (Residues: 38-271; Coverage: 100%)
Gene name: PRDX4
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSO 5 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 108.746Å b: 140.046Å c: 96.282Å
α: 90° β: 103.18° γ: 90°
R-values:
R R work R free
0.158 0.156 0.189
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling.
Perkins et al. (2015)
23 more

4 mentions without citation

Chemical approaches to detect and analyze protein sulfenic acids.
Furdui et al. (2014)
3 more