› 3tlu

X-ray diffraction
2.85Å resolution

The GLIC pentameric Ligand-Gated Ion Channel Loop2-24' oxidized mutant in a locally-closed conformation (LC1 subtype)

Released:
DOI: 10.2210/pdb3tlu/pdb
PDB entry 3tlu coloured by chain, front view.
PDB entry 3tlu coloured by chain, side view.
PDB entry 3tlu coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
A locally closed conformation of a bacterial pentameric proton-gated ion channel.
Prevost MS, Sauguet L, Nury H, Van Renterghem C, Huon C, Poitevin F, Baaden M, Delarue M, Corringer PJ
Nat Struct Mol Biol 19 642-9 (2012)
PMID: 22580559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 321 amino acids
Theoretical weight: 36.51 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 177.33Å b: 127.81Å c: 159.09Å
α: 90° β: 100.77° γ: 90°
R-values:
R R work R free
0.204 0.203 0.223
Expression system: Escherichia coli

Quick links

Citations

21 review citations

50 years of allosteric interactions: the twists and turns of the models.
Changeux JP. (2013)
20 more

4 mentions without citation

Propofol binding to the resting state of the gloeobacter violaceus ligand-gated ion channel (GLIC) induces structural changes in the inter- and intrasubunit transmembrane domain (TMD) cavities.
Ghosh et al. (2013)
3 more