› 3tmh

X-ray diffraction
3.8Å resolution

Crystal structure of dual-specific A-kinase anchoring protein 2 in complex with cAMP-dependent protein kinase A type II alpha and PDZK1

Released:
DOI: 10.2210/pdb3tmh/pdb
PDB entry 3tmh coloured by chain, front view.
PDB entry 3tmh coloured by chain, side view.
PDB entry 3tmh coloured by chain, top view.
Source organisms:
Entry authors: Sarma GN, Phan RH, Sankaran B, Taylor SS

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero icosamer (preferred)
PDBe Complex ID:
PDB-CPX-129007 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Na(+)/H(+) exchange regulatory cofactor NHE-RF3 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 87 amino acids
Theoretical weight: 9.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T2W1 (Residues: 375-459; Coverage: 16%)
Gene names: CAP70, NHERF3, PDZD1, PDZK1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: B, C, F, G
Molecule details ›
Chains: B, C, F, G
Length: 48 amino acids
Theoretical weight: 5.54 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12368 (Residues: 1-45; Coverage: 11%)
Gene name: Prkar2a
Sequence domains: Regulatory subunit of type II PKA R-subunit
A-kinase anchor protein 10, mitochondrial Chains: D, H, L
Molecule details ›
Chains: D, H, L
Length: 45 amino acids
Theoretical weight: 5.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43572 (Residues: 623-662; Coverage: 6%)
Gene name: AKAP10

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3221
Unit cell:
a: 121.472Å b: 121.472Å c: 88.282Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.268 0.328
Expression system: Escherichia coli

Quick links