› 3tsh

X-ray diffraction
1.9Å resolution

Crystal structure of Phl p 4, a grass pollen allergen with glucose dehydrogenase activity

Released:
DOI: 10.2210/pdb3tsh/pdb
PDB entry 3tsh coloured by chain, front view.
PDB entry 3tsh coloured by chain, side view.
PDB entry 3tsh coloured by chain, top view.
Source organism: Phleum pratense
Primary publication:
Crystal structure and immunologic characterization of the major grass pollen allergen Phl p 4.
Zafred D, Nandy A, Pump L, Kahlert H, Keller W
J Allergy Clin Immunol 132 696-703.e10 (2013)
PMID: 23683465

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 55.72 KDa
Source organism: Phleum pratense
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q2I6V7 (Residues: 26-525; Coverage: 99%)
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FDA 1 x FDA
Ligand NA 2 x NA
Ligand FMT 1 x FMT
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6122
Unit cell:
a: 117.077Å b: 117.077Å c: 202.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.162 0.19
Expression system: Komagataella pastoris

Quick links

Citations

15 citation in other articles

EAACI Molecular Allergology User's Guide.
Matricardi et al. (2016)
14 more

2 mentions without citation

Structure of allergens and structure based epitope predictions.
Dall'antonia et al. (2014)
1 more