› 3tt1

X-ray diffraction
3.1Å resolution

Crystal Structure of LeuT in the outward-open conformation in complex with Fab

Released:
DOI: 10.2210/pdb3tt1/pdb
PDB entry 3tt1 coloured by chain, front view.
PDB entry 3tt1 coloured by chain, side view.
PDB entry 3tt1 coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Krishnamurthy H, Gouaux E
Nature 481 469-74 (2012)
PMID: 22230955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208140 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Na(+):neurotransmitter symporter (Snf family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 58 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family
mouse monoclonal 1gG2a Fab fragment, heavy chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 218 amino acids
Theoretical weight: 23.91 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
mouse monoclonal 1gG2a Fab fragment, kappa light chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 219 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand SOG 5 x SOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 102.859Å b: 162.761Å c: 210.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.219 0.24
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

69 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
68 more

59 mentions without citation

The solute carrier 6 family of transporters.
Bröer et al. (2012)
58 more