› 3tt3

X-ray diffraction
3.22Å resolution

Crystal Structure of LeuT in the inward-open conformation in complex with Fab

Released:
DOI: 10.2210/pdb3tt3/pdb
PDB entry 3tt3 coloured by chain, front view.
PDB entry 3tt3 coloured by chain, side view.
PDB entry 3tt3 coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Krishnamurthy H, Gouaux E
Nature 481 469-74 (2012)
PMID: 22230955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208141 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 519 amino acids
Theoretical weight: 57.91 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 1-513; Coverage: 100%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family
mouse monoclonal 1gG1 Fab fragment, heavy chain Chain: L
Molecule details ›
Chain: L
Length: 218 amino acids
Theoretical weight: 24.21 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
mouse monoclonal 1gG1 Fab fragment, kappa light chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.99 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SOG 1 x SOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 128.838Å b: 169.804Å c: 130.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.257 0.3
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

69 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
68 more

73 mentions without citation

The solute carrier 6 family of transporters.
Bröer et al. (2012)
72 more