› 3tw5

X-ray diffraction
2.95Å resolution

Crystal structure of the GP42 transglutaminase from Phytophthora sojae

Released:
DOI: 10.2210/pdb3tw5/pdb
PDB entry 3tw5 coloured by chain, front view.
PDB entry 3tw5 coloured by chain, side view.
PDB entry 3tw5 coloured by chain, top view.
Source organism: Phytophthora sojae
Primary publication:
Structural and phylogenetic analyses of the GP42 transglutaminase from Phytophthora sojae reveal an evolutionary relationship between oomycetes and marine Vibrio bacteria.
Reiss K, Kirchner E, Gijzen M, Zocher G, Löffelhardt B, Nürnberger T, Stehle T, Brunner F
J Biol Chem 286 42585-42593 (2011)
PMID: 21994936

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transglutaminase elicitor Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 40.22 KDa
Source organism: Phytophthora sojae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q01928 (Residues: 163-529; Coverage: 73%)
Gene name: gpe1
Sequence domains: Transglutaminase elicitor
Structure domains: Transglutaminase elicitor, body domain

Ligands and Environments

1 bound ligand:
Ligand CXS 2 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P6222
Unit cell:
a: 195.48Å b: 195.48Å c: 137.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.215 0.234
Expression system: Komagataella pastoris

Quick links

Citations

7 review citations

Mechanisms and evolution of virulence in oomycetes.
Jiang et al. (2012)
6 more

4 mentions without citation

Structural and phylogenetic analyses of the GP42 transglutaminase from Phytophthora sojae reveal an evolutionary relationship between oomycetes and marine Vibrio bacteria.
Reiss et al. (2011)
3 more