› 3u11

X-ray diffraction
2.5Å resolution

Tetramerization dynamics of the C-terminus underlies isoform-specific cAMP-gating in HCN channels

Released:
DOI: 10.2210/pdb3u11/pdb
PDB entry 3u11 coloured by chain, front view.
PDB entry 3u11 coloured by chain, side view.
PDB entry 3u11 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels.
Lolicato M, Nardini M, Gazzarrini S, Möller S, Bertinetti D, Herberg FW, Bolognesi M, Martin H, Fasolini M, Bertrand JA, Arrigoni C, Thiel G, Moroni A
J Biol Chem 286 44811-20 (2011)
PMID: 22006928

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3Q4 (Residues: 521-723; Coverage: 17%)
Gene name: HCN4
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CMP 2 x CMP
Ligand GOL 5 x GOL
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P4
Unit cell:
a: 88.31Å b: 88.31Å c: 57.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.277
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels: from Biophysics to Pharmacology of a Unique Family of Ion Channels.
Sartiani et al. (2017)
8 more

4 mentions without citation

Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels.
Lolicato et al. (2011)
3 more