› 3u1k

X-ray diffraction
2.13Å resolution

Crystal structure of human PNPase

Released:
DOI: 10.2210/pdb3u1k/pdb
PDB entry 3u1k coloured by chain, front view.
PDB entry 3u1k coloured by chain, side view.
PDB entry 3u1k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human polynucleotide phosphorylase: insights into its domain function in RNA binding and degradation.
Lin CL, Wang YT, Yang WZ, Hsiao YY, Yuan HS
Nucleic Acids Res 40 4146-57 (2012)
PMID: 22210891

Function and Biology Details

Reaction catalysed: 
RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyribonucleotide nucleotidyltransferase 1, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 630 amino acids
Theoretical weight: 68.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TCS8 (Residues: 46-669; Coverage: 80%)
Gene names: PNPASE, PNPT1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 8 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: R3
Unit cell:
a: 289.753Å b: 289.753Å c: 92.785Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.223
Expression system: Escherichia coli

Quick links

Citations

13 review citations

MitomiRs delineating the intracellular localization of microRNAs at mitochondria.
Bandiera et al. (2013)
12 more

7 mentions without citation

Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase.
Falchi et al. (2022)
6 more