› 3u2e

X-ray diffraction
2.32Å resolution

EAL domain of phosphodiesterase PdeA in complex with 5'-pGpG and Mg++

Released:
DOI: 10.2210/pdb3u2e/pdb
PDB entry 3u2e coloured by chain, front view.
PDB entry 3u2e coloured by chain, side view.
PDB entry 3u2e coloured by chain, top view.
Source organism: Caulobacter vibrioides CB15
Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Massa C, Schirmer T, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189469 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
GGDEF family protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 28.75 KDa
Source organism: Caulobacter vibrioides CB15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A310 (Residues: 286-546; Coverage: 47%)
Gene name: CC_3396
Sequence domains: EAL domain
Structure domains: EAL domain
RNA (5'-R(P*GP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 2 nucleotides
Theoretical weight: 645 Da
Source organism: Caulobacter vibrioides CB15
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 62.144Å b: 75.154Å c: 129.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.237
Expression systems:
  • Escherichia coli
  • Not provided

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