3u43

X-ray diffraction
1.72Å resolution

Crystal structure of the colicin E2 DNase-Im2 complex

Released:
Source organism: Escherichia coli

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-137635 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E2 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04482 (Residues: 1-86; Coverage: 100%)
Gene names: ceiB, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Colicin E immunity protein
Colicin-E2 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04419 (Residues: 449-581; Coverage: 23%)
Gene names: ceaB, col
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 121.814Å b: 53.277Å c: 32.785Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.202
Expression system: Escherichia coli