› 3ua0

X-ray diffraction
3Å resolution

N-Terminal Domain of Bombyx mori Fibroin Mediates the Assembly of Silk in Response to pH Decrease

Released:
DOI: 10.2210/pdb3ua0/pdb
PDB entry 3ua0 coloured by chain, front view.
PDB entry 3ua0 coloured by chain, side view.
PDB entry 3ua0 coloured by chain, top view.
Source organism: Bombyx mori
Primary publication:
N-Terminal domain of Bombyx mori fibroin mediates the assembly of silk in response to pH decrease.
He YX, Zhang NN, Li WF, Jia N, Chen BY, Zhou K, Zhang J, Chen Y, Zhou CZ
J Mol Biol 418 197-207 (2012)
PMID: 22387468

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroin heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.14 KDa
Source organism: Bombyx mori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05790 (Residues: 1-126; Coverage: 2%)
Gene name: FIBH
Sequence domains: Fibroin heavy chain, N-terminal domain
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6522
Unit cell:
a: 74.52Å b: 74.52Å c: 208.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.312 0.311 0.336
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Advances in silkworm studies accelerated by the genome sequencing of Bombyx mori.
Xia et al. (2014)
8 more

3 mentions without citation

Towards Non-stick Silk: Tuning the Hydrophobicity of Silk Fibroin Protein.
Fountain et al. (2022)
2 more