› 3uap

X-ray diffraction
2.8Å resolution

Crystal structure of glutathione transferase (TARGET EFI-501774) from methylococcus capsulatus str. bath

Released:
DOI: 10.2210/pdb3uap/pdb
PDB entry 3uap coloured by chain, front view.
PDB entry 3uap coloured by chain, side view.
PDB entry 3uap coloured by chain, top view.
Source organism: Methylococcus capsulatus str. Bath
Entry authors: Patskovsky Y, Toro R, Bhosle R, Zencheck WD, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Imker HJ, Armstrong RN, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 25.88 KDa
Source organism: Methylococcus capsulatus str. Bath
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60CN1 (Residues: 1-203; Coverage: 100%)
Gene names: MCA0074, gst
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4332
Unit cell:
a: 129.246Å b: 129.246Å c: 129.246Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.259
Expression system: Escherichia coli BL21(DE3)

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