› 3ubr

X-ray diffraction
2.59Å resolution

Laue structure of Shewanella oneidensis cytochrome-c Nitrite Reductase

Released:
DOI: 10.2210/pdb3ubr/pdb
PDB entry 3ubr coloured by chain, front view.
PDB entry 3ubr coloured by chain, side view.
PDB entry 3ubr coloured by chain, top view.
Source organism: Shewanella oneidensis
Primary publication:
Laue crystal structure of Shewanella oneidensis cytochrome c nitrite reductase from a high-yield expression system.
Youngblut M, Judd ET, Srajer V, Sayyed B, Goelzer T, Elliott SJ, Schmidt M, Pacheco AA
J Biol Inorg Chem 17 647-62 (2012)
PMID: 22382353

Function and Biology Details

Reaction catalysed: 
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c-552 Chains: A, B
Molecule details ›
Chains: A, B
Length: 439 amino acids
Theoretical weight: 49.57 KDa
Source organism: Shewanella oneidensis
Expression system: Shewanella
UniProt:
  • Canonical: Q8EAC7 (Residues: 28-466; Coverage: 100%)
Gene names: SO_3980, nrfA
Sequence domains: Cytochrome c552
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 10 x HEC
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 51.5Å b: 95.9Å c: 223.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.19 0.285
Expression system: Shewanella

Quick links

Citations

4 review citations

Multi-heme proteins: nature's electronic multi-purpose tool.
Bewley et al. (2013)
3 more

5 mentions without citation

Multi-haem cytochromes in Shewanella oneidensis MR-1: structures, functions and opportunities.
Breuer et al. (2015)
4 more