› 3ug9

X-ray diffraction
2.3Å resolution

Crystal Structure of the Closed State of Channelrhodopsin

Released:
DOI: 10.2210/pdb3ug9/pdb
PDB entry 3ug9 coloured by chain, front view.
PDB entry 3ug9 coloured by chain, side view.
PDB entry 3ug9 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Crystal structure of the channelrhodopsin light-gated cation channel.
Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, Ishitani R, Deisseroth K, Nureki O
Nature 482 369-74 (2012)
PMID: 22266941

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Archaeal-type opsin 1; Sensory opsin B Chain: A
Molecule details ›
Chain: A
Length: 333 amino acids
Theoretical weight: 37.44 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q93WP2 (Residues: 24-245; Coverage: 32%)
  • Canonical: Q8RUT8 (Residues: 207-309; Coverage: 14%)
Gene names: CHLRE_02g085257v5, CSOB, acop1, acop2, cop4
Sequence domains: Bacteriorhodopsin-like protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand OLA 6 x OLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2221
Unit cell:
a: 59.471Å b: 139.114Å c: 90.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.204 0.251
Expression system: Spodoptera frugiperda

Quick links

Citations

56 review citations

Optogenetic investigation of neural circuits underlying brain disease in animal models.
Tye et al. (2012)
55 more

57 mentions without citation

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
56 more