› 3ugo

X-ray diffraction
2.1Å resolution

Crystal structure of RNA-polymerase sigma subunit domain 2 complexed with -10 promoter element ssDNA oligo (TACAAT)

Released:
DOI: 10.2210/pdb3ugo/pdb
PDB entry 3ugo coloured by chain, front view.
PDB entry 3ugo coloured by chain, side view.
PDB entry 3ugo coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Structural basis for promoter-10 element recognition by the bacterial RNA polymerase σ subunit.
Feklistov A, Darst SA
Cell 147 1257-69 (2011)
PMID: 22136875

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-116984 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA polymerase sigma factor SigA Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.04 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EZJ8 (Residues: 92-332; Coverage: 55%)
Gene name: sigA
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
5'-D(*TP*GP*TP*AP*CP*AP*AP*TP*GP*GP*G)-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand K 5 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P422
Unit cell:
a: 86.134Å b: 86.134Å c: 111.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.237
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

38 review citations

Bacterial sigma factors: a historical, structural, and genomic perspective.
Feklístov et al. (2014)
37 more

11 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
10 more