› 3ugv

X-ray diffraction
2.3Å resolution

Crystal structure of an enolase from alpha pretobacterium bal199 (EFI TARGET EFI-501650) with bound MG

Released:
DOI: 10.2210/pdb3ugv/pdb
PDB entry 3ugv coloured by chain, front view.
PDB entry 3ugv coloured by chain, side view.
PDB entry 3ugv coloured by chain, top view.
Source organism: alpha proteobacterium BAL199
Entry authors: Vetting MW, Toro R, Bhosle R, Wasserman SR, Morisco LL, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108787 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 390 amino acids
Theoretical weight: 42.94 KDa
Source organism: alpha proteobacterium BAL199
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A8TYI5 (Residues: 1-367; Coverage: 100%)
Gene name: BAL199_22462
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 16 x MG
Ligand CL 3 x CL
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 152.711Å b: 152.984Å c: 173.535Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.208
Expression system: Escherichia coli BL21(DE3)

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